生物学杂志

生物学杂志 ›› 2022, Vol. 39 ›› Issue (1): 62-.doi: 10. 3969/ j. issn. 2095-1736. 2022. 01. 062

• 研究报告 • 上一篇    下一篇

乌拉尔甘草CIPK 基因家族鉴定与表达分析

  

  1. 石河子大学生命科学学院 新疆植物药资源利用教育部重点实验室, 石河子832003
  • 出版日期:2022-02-18 发布日期:2022-02-15
  • 通讯作者: 李鸿彬,博士,教授,研究方向为植物分子生物学与基因工程,E-mail:lihb@shzu.edu.cn
  • 作者简介:仙亚杰,硕士研究生,研究方向为生物化学与分子生物学,E-mail:1029718818@qq.com
  • 基金资助:
    新疆生产建设兵团科技计划项目 (2018AB012,2020AA005)

Genome-wide identification and expression analysis of CIPK gene family in Glycyrrhiza uralensis

  1. Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education,College of Life Sciences, Shihezi University, Shihezi 832003, China
  • Online:2022-02-18 Published:2022-02-15

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摘要：

为挖掘乌拉尔甘草钙调磷酸酶B 互作蛋白激酶 (Calcineurin B like protein interacting protein kinase, CIPK)家族成员特征并预测其功能,利用生物信息学方法鉴定乌拉尔甘草CIPK 家族基因,并对其进行系统发育、基因结构、保守结构域及进化关系、表达特征等分析。从乌拉尔甘草基因组中鉴定获得22 个GuCIPK 基因,分布于19 个不同的染色体位置;氨基酸序列比对分析发现多数GuCIPK 都含有1 个Ser/ Thr 结构域、1 个NAF 结构域以及PPI 结构域;进化树分析结果显示,22 个GuCIPK 基因可分为5 个亚组。GuCIPK 基因的上游启动子中存在一些非生物胁迫和激素应答元件,推测GuCIPK 基因的功能发挥可能受不同信号的调控。根据组织特异性表达特征可将GuCIPK 分为Ⅰ~Ⅳ 4 个组,其中 Ⅱ 组中6 个GuCIPK 成员在根、茎和叶片中的表达量较高,Ⅰ、Ⅲ、Ⅳ组的GuCIPK 成员在根、茎、叶组织中的表达相对较低。利用qRT-PCR 分GuCIPK 响应不同非生物胁迫的表达特征,结果表明:在盐、氧化、干旱、高温和低温胁

迫下,GuCIPK 基因受到不同胁迫的诱导表达。结果为进一步探究GuCIPK 的功能和可能的调控机制提供了一定参考。


关键词: 乌拉尔甘草, CIPK, 基因家族鉴定, 基因表达分析, 非生物胁迫

Abstract:

In order to explore the characteristics of CIPK family members and predict their function, the CIPK family genes of Glycyrrhiza uralensis were identified by bioinformatics, and their phylogeny, gene structure, conserved motif and evolutionary relationship were analyzed. The results showed that 22 GuCIPK genes were identified from Glycyrrhiza uralensis, and chromosomal location analysis showed that they were distributed on 19 different scaffolds. Amino acid sequence alignment indicated that most of the GuCIPKs contained a Ser/ Thr domain, a NAF domain and a PPI domain. Phylogenetic tree analysis displayed that the 22 GuCIPKs could be classified into 5 subfamilies, and there existed multiple parallel homologous gene pairs between GuCIPKs and CIPKs of Arabidopsis thaliana and Glycine max. G. uralensis GuCIPK promoter regions included some cis-elements involved in response to abiotic stress and hormone,suggesting their potential functions under regulation of different signals. Tissue-specific expression pattern analysis showed that the GuCIPKs could be classified into four groups Ⅰ-Ⅳ, and the six members of group Ⅱ had high accumulations in roots, stems and leaves, while the other GuCIPK members of groups Ⅰ, Ⅲ and Ⅳ three groups appeared relative low abundance in the tissues of root, stem and leaf. Quantitative real time PCR (qRT-PCR) analysis demonstrated that GuCIPK genes were induced significantly by different abiotic stresses of salt, oxidation, drought, high temperature and low temperature. These results provide effective reference to investigate the GuCIPK function and elucidate the possible regulatory mechanism.


Key words: Glycyrrhiza uralensis, CIPK, gene family identification, gene expression analysis, abiotic stress

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